Year 2019 / Volume 111 / Number 2
Original
Detection and quantification of gluten immunogenic peptides in feces of infants and their relationship with diet

106-110

DOI: 10.17235/reed.2018.5549/2018

María Roca, Ester Donat, Etna Masip, Paula Crespo Escobar, Victoria Fornes-Ferrer, Begoña Polo, Carmen Ribes-Koninckx,

Abstract
Background: there are no effective methods to easily control the correct adherence to a gluten-free diet (GFD) in celiac disease (CD) patients. Aim: to assess the sensitivity and specificity of a rapid immunochromatographic (IC) test that detects gluten immunogenic peptides (GIP) in feces, compared to an enzyme-linked immunosorbent assay (ELISA) method. Methods: fecal samples from healthy infants were analyzed by a rapid IC test and ELISA, both methods are based on the anti-gliadin 33-mer monoclonal antibody. Group 1 included infants aged from 6 to 24 months, with an unrestricted consumption of gluten containing cereals. Group 2 (negative controls) was comprised of infants aged from 0 to 6 months, either breastfed or formula fed who had never ingested gluten. Results: in group 1 (n = 34), all infants had positive values by ELISA, the mean was 13.13 μgGIP/g (range 0.56-46.79). The IC test was negative in 5/20 cases and there was a significant correlation (p=0.006) between the mean daily gluten intake and GIP in feces. In group 2 (n = 20), all the samples were negative by both methods. Moreover, the Kappa Fleiss concordance index (Kappa = 0.79 CI95% [0.616, 0.965]) indicated a moderate concordance between both methods. Conclusions: according to our results, both methods are highly specific. However, the ELISA test had a higher sensitivity. Although we found a significant correlation between the amount of gluten consumed and GIP recovery in feces, further studies are needed to clarify the impact of individual confounding factors in GIP recovery.
Share Button
New comment
Comments
No comments for this article
References
1. Monzani A, Rapa A, Fonio P, et al. Use of deamidated gliadin peptide antibodies to monitor diet compliance in childhood celiac disease. J Pediatr Gastroenterol Nutr 2011;53(1):55-60.
2. Comino I, Fernández-Bañares F, Esteve M, et al. Fecal Gluten Peptides Reveal Limitations of Serological Tests and Food Questionnaires for Monitoring Gluten-Free Diet in Celiac Disease Patients. Am J Gastroenterol 2016;111(10):1456-65.
3. Duerksen DR, Wilhelm-Boyles C, Parry DM. Intestinal permeability in long-term follow-up of patients with celiac disease on a gluten-free diet. Dig Dis Sci 2005;50(4):785–90.
4. Ertekin V, Selimoğlu MA, Turgut A, et al. Fecal calprotectin concentration in celiac disease. J Clin Gastroenterol 2010;44(8):544–6.
5. Planas R, Pujol-Autonell I, Ruiz E, et al. Regenerating gene Iα is a biomarker for diagnosis and monitoring of celiac disease: a preliminary study. Transl Res 2011;158(3):140–5.
6. Lind MV, Madsen ML, Rumessen JJ, et al. Plasma alkylresorcinols reflect gluten intake and distinguish between gluten-rich and gluten-poor diets in a population at risk of metabolic syndrome. J Nutr 2016;146(10):1991–8.
7. Capone P, Rispo A, Imperatore N, et al. Fecal calprotectin in coeliac disease. World J Gastroenterol 2014;20(2):611–2.
8. Duerksen DR, Wilhelm-Boyles C, Veitch R, et al. A comparison of antibody testing, permeability testing, and zonulin levels with small-bowel biopsy in celiac disease patients on a gluten-free diet. Dig Dis Sci 2010;55(44):1026–31.
9. Adriaanse M, Leffler DA. Serum markers in the clinical management of celiac disease. Dig Dis 2015;33(2):236–43.
10. Kaukinen K, Peräaho M, Lindfors K, et al. Persistent small bowel mucosal villous atrophy without symptoms in coeliac disease. Aliment Pharmacol Ther 2007;25(10):1237–45.
11. Kaukinen K, Sulkanen S, Mäki M, et al. IgA-class transglutaminase antibodies in evaluating the efficacy of gluten-free diet in coeliac disease. Eur J Gastroenterol Hepatol 2002;14(3):311–5.
12. Tursi A, Brandimarte G, Giorgetti GM. Lack of usefulness of anti-transglutaminase antibodies in assessing histologic recovery after gluten-free diet in celiac disease. J Clin Gastroenterol 2003;37(5):387–91.
13. Tursi A, Brandimarte G, Giorgetti, GM, et al. Endoscopic and histological findings in the duodenum of adults with celiac disease before and after changing to a gluten-free diet: A 2-year prospective study. Endoscopy 2006;38(7):702–7.
14. Biagi F, Campanella J, Martucci S, et al. A milligram of gluten a day keeps the mucosal recovery away: A case report. Nutr Rev 2004;62(9):360–3.
15. Rashtak S, Ettore MW, Homburger HA, et al. Comparative usefulness of deamidated gliadin antibodies in the diagnosis of celiac disease. Clin Gastroenterol Hepatol 2008;6(4):426–32.
16. Vallejo-Diez S, Bernardo D, Moreno ML, et al. Detection of specific IgA antibodies against a novel deamidated 8-Mer gliadin peptide in blood plasma samples from celiac patients. PLoS ONE 2013;8(11):e80982.
17. Morón B, Bethune MT, Comino I, et al. Toward the assessment of food toxicity for celiac patients: Characterization of monoclonal antibodies to a main immunogenic gluten peptide. PLoS ONE 2008;3(5):e2294.
18. Comino I, Real A, Vivas S, et al. Monitoring of gluten-free diet compliance in celiac patients by assessment of gliadin 33-mer equivalent epitopes in feces. Am J Clin Nutr 2012;95(3):670-7.
19. Moreno ML, Cebolla Á, Muñoz-Suano A, et al. Detection of gluten immunogenic peptides in the urine of patients with coeliac disease reveals transgressions in the gluten-free diet and incomplete mucosal healing. Gut 2017;66(2):250-7.
20. Overbeek FM, Uil-Dieterman IG, Mol IW, et al. The daily gluten intake in relatives of patients with coeliac disease compared with that of the general Dutch population. Eur J Gastroenterol Hepatol 1997;9(11):1097–9.
21. Peter Koehler, editor. Analysis of gluten in human milk samples in coeliac and non-coeliac mothers. Proceedings of the 26th Meeting of the Working Group on Prolamin Analysis and Toxicity: 2013 39-44: Leuven, Belgium: Verlag Wissenschaftliche Scripten; 2013.
22. Chirdo FG, Rumbo M, Anon MC, et al. Presence of high levels of non-degraded gliadin in breast milk from healthy mothers. Scand J Gastroenterol 1998;33(11):1186-92.
23. Troncone R, Scarcella A, Donatiello A, et al. Passage of gliadin into human breast milk. Acta Paediatr Scand 1987;76(3):453-6.
Related articles

Letter

Liver disorders and celiac disease

DOI: 10.17235/reed.2023.9516/2023

Digestive Diseases Image

Calcified cavitating mesenteric lymph node syndrome: a rare complication of celiac disease

DOI: 10.17235/reed.2020.7494/2020

Letter

Balanced by iron. Hereditary hemochromatosis and celiac disease

DOI: 10.17235/reed.2020.7269/2020

Review

New celiac disease biomarkers

DOI: 10.17235/reed.2020.7217/2020

Editorial

Is celiac disease really associated with inflammatory bowel disease?

DOI: 10.17235/reed.2019.6779/2019

Original

Coping with celiac disease: how heavy is the burden for caregivers?

DOI: 10.17235/reed.2017.4518/2016

Letter to the Editor

Celiac disease and fibromyalgia: Is there an association?

DOI: 10.17235/reed.2015.3992/2015

Citation tools
Roca M, Donat E, Masip E, Crespo Escobar P, Fornes-Ferrer V, Polo B, et all. Detection and quantification of gluten immunogenic peptides in feces of infants and their relationship with diet. 5549/2018


Download to a citation manager

Download the citation for this article by clicking on one of the following citation managers:

Metrics
This article has received 1301 visits.
This article has been downloaded 304 times.

Statistics from Dimensions


Statistics from Plum Analytics

Publication history

Received: 19/02/2018

Accepted: 08/08/2018

Online First: 15/10/2018

Published: 01/02/2019

Article revision time: 163 days

Article Online First time: 238 days

Article editing time: 347 days


Share
This article has been rated by 3 readers.
Reader rating:
Valora este artículo:




Asociación Española de Ecografía Digestiva Sociedad Española de Endoscopia Digestiva Sociedad Española de Patología Digestiva
The Spanish Journal of Gastroenterology is the official organ of the Sociedad Española de Patología Digestiva, the Sociedad Española de Endoscopia Digestiva and the Asociación Española de Ecografía Digestiva
Cookie policy Privacy Policy Legal Notice © Copyright 2023 y Creative Commons. The Spanish Journal of Gastroenterology