Year 2022 / Volume 114 / Number 9
Letter
Altered Expression of TMEM16A in Colorectal Carcinoma and Its Clinical Significance

560-561

DOI: 10.17235/reed.2022.8756/2022

Fang He, Jia-Jia Liu, Shi-Bin Guo,

Abstract
Dear Editor, TMEM16A has been reported to be over-expressed in some malignant tumors recently[1]. The aim of this study was to investigate the role of TMEM16A in the progression of colorectal carcinoma (CRC) by detecting the expression of TMEM16A in CRC tissues, adjacent normal tissues, and adenoma tissues. Then, to investigate the relationship between TMEM16A expression and the clinicopathological features of patients with CRC. 40 cases of CRC and 40 cases of adenoma specimen from July 2016 to November 2016 were enrolled in this retrospective study. Adjacent normal tissue was defined as the area more than 5cm away from the cancer tissue. Expression of TMEM16A mRNA in CRC tissues and adjacent tissues was detected by real-time PCR, while protein expression was determined by immunohistochemical analysis and western blot. The results showed that the expression of TMEM16A in CRC tissues was higher than that in adenoma tissues (P =0.025) and adjacent tissues (P<0.001), and the expression of TMEM16A in adenoma tissues was higher than that in adjacent normal tissues (P=0.041), which was consistent with previous reports about CRC cells[2], and indicated that TMEM16A may be associated with the progression of normal colonic epithelium into adenoma and into adenocarcinoma[3]. 40 cases of CRC were divided into groups according to gender, age, degree of differentiation, depth of invasion, TNM stage, distant metastasis, lymph node metastasis, serum CEA level, gross classification and tumor location. The expression of TMEM16A was higher in the group of TNM stage III + IV than that in the group of I + II stage, was higher in the distant metastasis group than the non-distant metastasis group, was higher in the lymph node metastasis group than the no lymph node metastasis group, was higher in the serum CEA increased group than the normal serum CEA group (P< 0.05), which indicated that over-expression of TMEM16A was associated with increased potentiality of metastasis. The results were consistent with previous reports with CRC cell lines[4]. Our previous study also showed that upregulation of TMEM16A protein expression is an independent predictive factor for poor prognosis in patients with CRC by multivariate Cox regression analysis (P<0.05, RR=6.467, 95% CI: 1.777- 23.538)[5]. TMEM16A has potential as a therapeutic target for CRC patients.
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References
1、 Duvvuri U, Shiwarski DJ, Xiao D, Bertrand C, Huang X, Edinger RS, Rock JR, Harfe BD, Henson BJ, Kunzelmann K et al: TMEM16A induces MAPK and contributes directly to tumorigenesis and cancer progression. Cancer Res 2012, 72(13):3270-3281.
2、 Sui Y, Sun M, Wu F, Yang L, Di W, Zhang G, Zhong L, Ma Z, Zheng J, Fang X et al: Inhibition of TMEM16A expression suppresses growth and invasion in human colorectal cancer cells. PLoS One 2014, 9(12):e115443.
3、 Stoffel EM, Boland CR: Genetics and Genetic Testing in Hereditary Colorectal Cancer. Gastroenterology 2015, 149(5):1191-1203 e1192.
4、 Park YR, Lee ST, Kim SL, Zhu SM, Lee MR, Kim SH, Kim IH, Lee SO, Seo SY, Kim SW: Down-regulation of miR-9 promotes epithelial mesenchymal transition via regulating anoctamin-1 (ANO1) in CRC cells. Cancer Genet 2019, 231-232:22-31.
5、Jia-Jia Liu, Fang He, Shi-Bin Guo: TMEM16A overexpression indicates poor prognosis in colorectal cancer patients. Rev Esp Enferm Dig 2021 Oct 5.
DOI: 10.17235/reed.2021.8292/2021
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He F, Liu J, Guo S. Altered Expression of TMEM16A in Colorectal Carcinoma and Its Clinical Significance. 8756/2022


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Publication history

Received: 27/02/2022

Accepted: 29/03/2022

Online First: 04/04/2022

Published: 07/09/2022

Article revision time: 25 days

Article Online First time: 36 days

Article editing time: 192 days


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