Year 2018 / Volume 110 / Number 1
Original
Serum thymosin beta4 as a noninvasive biomarker in patients with nonalcoholic steatohepatitis

19-24

DOI: 10.17235/reed.2017.4690/2016

Yong Jiang, Tao Han, Zhi-Guang Zhang, Ying Zhang, Feng-Xiang Qi,

Abstract
Objective: The aim of the study was to determine whether serum thymosin beta4 (Tβ4) can be a useful noninvasive biomarker to differentiate between nonalcoholic steatohepatitis (NASH) and nonalcoholic fatty liver (NAFL). Methods: The study included 24 NAFL patients and 21 NASH patients. The levels of Tβ4, 8-hydroxydeoxyguanosine acid (8-OhdG), liver function parameters, blood lipid, and glucose were detected in the venous blood of all patients. The NAFLD histological activity score (NAS) was examined in biopsy specimens from all patients. Statistical analysis was performed in order to find differences between the two abovementioned groups. In addition, receiver operator characteristic (ROC) analyses for alanine aminotransferase (ALT) and Tβ4 levels were performed in NAFL and NASH patients and the cut-off value was determined. Associations between the variables were tested using correlation coefficient calculations. Statistical significance was set at a p value of < 0.05. Results: Serum Tβ4 content was 5.12 ± 1.87 mg/l in the NAFL group and 2.98 ± 1.35 mg/l in the NASH group (p < 0.001). Serum Tβ4 content and NAS, histological features of hepatic steatosis, lobular inflammation and ballooning, ALT, glucose and 8-OhdG levels were negatively correlated (p < 0.05 for all) in the NASH group. The correlation coefficient values were -0.530, -0.562, -0.574, -0.438, -0.446, -0.426 and -0.563, respectively. On the basis of ROC analysis, the best predictive Tβ4 cut-off value for detecting NASH was 3.94 mg/l (85.7% sensitivity and 79.2% specificity, which were higher than those of ALT). Conclusion: Serum Tβ4 level can be used as a biomarker for the diagnosis of NASH and was negatively correlated with the oxidation state of the liver.
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References
1. Ratziu V, Giral P, Charlotte F, Bruckert E, Thibault V, Theodorou I, Khalil L, Turpin G, Opolon P, Poynard T. Liver fibrosis in overweight patients. Gastroenterology2000;118: 1117–23
2. Oda K, Uto H, Mawatari S, Ido A. Clinical features of hepatocellular carcinoma associated with nonalcoholic fatty liver disease: a review of human studies. Clin J Gastroenterol2015, 8(1):1-9
3. Castera L, Vilgrain V, Angulo P. Noninvasive evaluation of NAFLD. Nat Rev Gastroenterol Hepatol.2013;10: 666-75
4. Ballweber E, Hannappel E, Huff T, Stephan H, Haener M, Taschner N, Stoffler D, Aebi U, Mannherz HG. Polymerization of chemically cross-linked actin:thymosin beta(4) complex to filamentous actin: alteration in helical parameters and visualization of thymosin beta(4) binding on F-actin.J Mol Biol 2002;315:613-25
5. Gupta S, Kumar S, Sopko N, Qin Y, Wei C, Kim IK. Thymosin β4 and cardiac protection: implication in inflammation and fibrosis. Ann N Y Acad Sci2012,1269:84-91
6. Qiu P,Wheater MK,Qiu Y, Sosne G..Thymosin beta4 inhibits TNF-alpha -induced NF-kappaB activation, IL-8 expression, and the sensitizing effects by its partners PINCH-1 and ILK. FASEB J2011;25:1815-1826
7. Dong QY,Han T,Wang LF,Dong YP, Liu Y,Wang B.The significance of serum thymosin β4 levels in patients with non-alcoholic fatty liver disease[J].Tianjin Medical Journal2013; 41:97-100
8. Sanyal AJ, Brunt EM, Kleiner DE, Kowdley KV, Chalasani N, Lavine JE, Ratziu V, McCullough A. Endpoints and clinical trial design for nonalcoholic steatohepatitis. Hepatology 2011;54:344-53
9. Rafiq N, Bai C, Fang Y, Srishord M, McCullough A, Gramlich T, Younossi ZM. Long-term follow-up of patients with no-nalcoholic fatty liver. Clin Gastroenterol Hepatol 2009;7:234-8
10. Shen J, Chan HL, Wong GL, Choi PC, Chan AW, Chan HY, Chim AM, Yeung DK, Chan FK, Woo J, Yu J, Chu WC, Wong VW. Non-invasive diagnosis of non-alcoholic steatohepatitis by combined serum biomarkers. J Hepatol 2012;56:1363-70.
11. Bell LN, Theodorakis JL, Vuppalanchi R, Saxena R, Bemis KG, Wang M, Chalasani N. Serum proteomics and biomarker discovery across the spectrum of nonalcoholic fatty liver disease. Hepatology2010; 51: 111-20
12. Adams LA, Talwalkar JA. Diagnostic evaluation of nonalcoholic fatty liver disease. J Clin Gastroenterol 2006; 40 Suppl 1: S34-S38
13. Yoneda M, Mawatari H, Fujita K, Iida H, Yonemitsu K, KatoS, Takahashi H, Kirikoshi H, Inamori M, Nozaki Y, Abe Y,Kubota K, Saito S, Iwasaki T, Terauchi Y, Togo S, MaeyamaS, Nakajima A. High-sensitivity C-reactive protein is an independent clinical feature of nonalcoholic steatohepatitis(NASH) and also of the severity of fbrosis in NASH. J Gas-troenterol 2007; 42: 573-82
14. Fierbinteanu-Braticevici C, Dina I, Petrisor A, Tribus L, Negreanu L, Carstoiu C. Noninvasive investigations for non alcoholic fatty liver disease and liver fibrosis. World J Gastroen-terol 2010; 16: 4784-91
15. Maleki I, Rastgar A, Hosseini V, Taghvaei T, Rafei A, Barzin M, Torabizadeh Z, Naghshvar F, Khalilian A. High sensitive CRP and pentraxine 3 as noninvasive biomarkers of nonalcoholic fatty liver disease. Eur Rev for Med Pharmacol Sci 2014; 18: 1583-90
16. Hu KC, Wang HY, Liu SC, Liu CC, Hung CL, Bair MJ, Liu CJ, Wu MS, Shih SC. Nonalcoholic fatty liver disease: Updates in noninvasive diagnosis and correlation with cardiovascular disease. World J Gastroenterol 2014; 20(24): 7718-29
17. Rafq N, Bai C, Fang Y, Srishord M, McCullough A, Gramlich T, Younossi ZM. Long-term follow-up of patients with nonalcoholic fatty liver.Clin Gastroenterol Hepatol 2009; 7: 234-8
18. Jamali R, Khonsari M, Merat S, Khoshnia M, Jafari E, Bahram Kalhori A, Abolghasemi H, Amini S, Maghsoudlu M, Deyhim MR, Rezvan H, Pourshams A. Persistent alanine aminotransferase elevation among the general Iranian population: prevalence and causes.World J Gastroenterol 2008; 14: 2867-71
19. Lee JY, Kim KM, Lee SG, Yu E, Lim YS, Lee HC, Chung YH, Lee YS, Suh DJ. Prevalence and risk factors of non-alcoholic fatty liver disease in potential living liver donors in Korea: a review of 589 consecutive liver biopsies in a single center. J Hepatol 2007; 47:239-4
20. Hui AY, Wong VW, Chan HL, Liew CT, Chan JL, Chan FK, Sung JJ. Histological progression of non-alcoholic fatty liver disease in Chinese patients. Aliment Pharmacol Ther 2005;21: 407-13
21. Leite NC, Salles GF, Araujo AL, VillelaNogueira CA, Cardoso CR. Prevalence and associated factors of non-alcoholic fatty liver disease in patients with type2 diabetes mellitus. Liver Int 2009; 29:113-9
22. Tian YY,Wang YB,Tan LL,Wang YY,Chen Y. value of Tβ4 liver function of non-alcoholic liver disease. Chin Gastroenterol Hepatol2014;23:432-4
23. Day CP, James OF. Steatohepatitis: a tale of two“hits”? Gastroenterology1998; 114: 842-5
24. Krawczyk M, Bonfrate L, Portincasa P. Nonalcoholic fatty liver disease. Best Pract Res Clin Gastroenterol 2010; 24: 695-708
25. Ishikawa H, Takaki A, Tsuzaki R, Yasunaka T, Koike K, Shimomura Y, Seki H, Matsushita H, Miyake Y, Ikeda F, Shiraha H, Nouso K,Yamamoto K. L-Carnitine Prevents Progression of Non-Alcoholic Steatohepatitis in a Mouse Model with Upregulation of Mitochondrial Pathway. PLoS One. 2014, 9: e100627
26. Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: How are they linked? Free Radic Biol Med2010:1603-16
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Jiang Y, Han T, Zhang Z, Zhang Y, Qi F. Serum thymosin beta4 as a noninvasive biomarker in patients with nonalcoholic steatohepatitis. 4690/2016


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Publication history

Received: 24/10/2016

Accepted: 21/04/2017

Online First: 18/12/2017

Published: 12/01/2018

Article revision time: 167 days

Article Online First time: 420 days

Article editing time: 445 days


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