Year 2019 / Volume 111 / Number 4
Original
Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models

301-307

DOI: 10.17235/reed.2019.6083/2018

Rocío Gallego-Durán, Leticia Álvarez-Amor, Antonio Gil-Gómez, Ángela Rojas, Rocío Muñoz-Hernández, Antonio Cádernas-García, Douglas Maya-Miles, Rocío Montero-Vallejo, Sheila Gato, Yolanda Sánchez Torrijos, Javier Ampuero, Francisco Martín, Manuel Romero-Gómez,

Abstract
Introduction: non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. Material and methods: this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated. Results: HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. Conclusion: in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing.
Share Button
New comment
Comments
No comments for this article
Related articles

Original

Implementation of a peroral endoscopic myotomy program

DOI: 10.17235/reed.2020.7116/2020

Citation tools
Gallego-Durán R, Álvarez-Amor L, Gil-Gómez A, Rojas Á, Muñoz-Hernández R, Cádernas-García A, et all. Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models. 6083/2018


Download to a citation manager

Download the citation for this article by clicking on one of the following citation managers:

Metrics
This article has received 1163 visits.
This article has been downloaded 441 times.

Statistics from Dimensions


Statistics from Plum Analytics

Publication history

Received: 30/11/2018

Accepted: 06/03/2019

Online First: 21/03/2019

Published: 04/04/2019

Article revision time: 85 days

Article Online First time: 111 days

Article editing time: 125 days


Share
This article has been rated by 31 readers.
Reader rating:
Valora este artículo:




Asociación Española de Ecografía Digestiva Sociedad Española de Endoscopia Digestiva Sociedad Española de Patología Digestiva
The Spanish Journal of Gastroenterology is the official organ of the Sociedad Española de Patología Digestiva, the Sociedad Española de Endoscopia Digestiva and the Asociación Española de Ecografía Digestiva
Cookie policy Privacy Policy Legal Notice © Copyright 2023 y Creative Commons. The Spanish Journal of Gastroenterology