Year 2016 / Volume 108 / Number 2
Original
Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease

71-78

DOI: 10.17235/reed.2015.3960/2015

Dingyang Li, Xiaoju Shi, Lijing Zhao, Zuowen Liang, Shuli Xie, Guangyi Wang,

Abstract
Background and objective: Polycystic liver disease (PCLD) represents a group of genetic disorders that include autosomal dominant polycystic kidney disease (ADPKD) and isolated polycystic liver disease (iPCLD). There is currently no definitive treatment except for liver transplantation. The aim of this study was to assess the expression level of aquaporin 1 (AQP1) on the PCLD cysts with different sizes and provide the potential therapeutic target. Methods: We collected 3 normal bile ducts, and recruited 8 patients with simple liver cyst disease, 24 patients with ADPKD, and 17 patients with iPCLD. AQP1 expression in different types of cyst walls and in normal bile ducts was detected using real time quantitative PCR, western blot and immunofluorescence staining. We also compared AQP1 expression levels in cysts of different sizes. Besides, ionic concentrations, pH and osmolality of cyst fluid were analyzed. Results: The results showed that AQP1 expression in PCLD cysts was significantly higher than that in simple liver cysts and the normal bile ducts. In addition, a comparable increasing trend was found in cysts of smaller sizes to cysts of larger sizes. pH values, the sodium and chloride concentrations were higher in cyst fluid than that in the serum. Conclusions: AQP1 was overexpressed in cystic cholangiocytes. A tendency of increased AQP1 protein expression in correlation with the cyst size was also found. These observations offered a direction into the molecular mechanisms of cyst expansion and maybe provide new treatment strategies to reduce fluid secretion into liver cysts.
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Li D, Shi X, Zhao L, Liang Z, Xie S, Wang G, et all. Overexpression of Aquaporin 1 on cysts of patients with polycystic liver disease. 3960/2015


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Publication history

Received: 07/08/2015

Accepted: 09/11/2015

Online First: 30/11/2015

Published: 01/02/2016

Article revision time: 91 days

Article Online First time: 115 days

Article editing time: 178 days


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